Science Advances

Supplementary Materials

This PDF file includes:

  • fig. S1. T-coffee analysis of mEAK-7 in eukaryotes, validation of human mEAK-7 antibody, and an expanded cell screen for mEAK-7 protein.
  • fig. S2. Extended immunofluorescence analysis of HA–mEAK-7WT in other cellular compartments.
  • fig. S3. Validation of mEAK-7 antibody for immunofluorescence analysis of endogenous mEAK-7.
  • fig. S4. mEAK-7 regulates serum-mediated activation of mTORC1 signaling and knockdown of mEAK-7 results in increased (Thr389) p-S6K1 levels.
  • fig. S5. Densitometry analysis of Fig. 1I.
  • fig. S6. Densitometry analysis of Fig. 1J.
  • fig. S7. Immunofluorescence analysis of endogenous mEAK-7 colocalizing with endogenous mTOR in response to nutrients and immunoprecipitation analysis of HA–mEAK-7 mutants for mTOR interaction.
  • fig. S8. Densitometry analysis of Fig. 4.
  • fig. S9. Knockdown of mEAK-7 does not result in enhanced cell apoptosis but increases cell size.
  • table S1. Cloning primers.
  • Extended Materials and Methods for cloning.

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