Science Advances

Supplementary Materials

This PDF file includes:

  • Supplementary Materials and Methods
  • fig. S1. Na+-dependent binding of 3Hleucine to purified LeuT.
  • fig. S2. Correlation between the measured HDX and secondary structure elements in LeuT.
  • fig. S3. Deuterium uptake plots for detergent-solubilized LeuT.
  • fig. S4. Impact of different leucine concentrations on local HDX rates in LeuT.
  • fig. S5. Partial unwinding of individual helices in LeuT reconstituted into phospholipid-containing bilayer nanodiscs.
  • fig. S6. Sequence coverage map for LeuT reconstituted into phospholipid-containing bilayer nanodiscs.
  • fig. S7. Na+- and substrate-induced stabilization of TM helices.
  • fig. S8. Compared conformations of EX1 segments in crystal structures.
  • table S1. RMSD and hydrogen bond variations between three x-ray structures of LeuT for the EX1 segments.
  • table S2. Detailed hydrogen bond analysis in three x-ray structures of LeuT for backbone amide groups in the EX1 segments.

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