Science Advances

Supplementary Materials

This PDF file includes:

  • fig. S1. Incorporation of the fluorescent dyes into σ70 and σB subunits.
  • fig. S2. Activity of the σ70 and σB double-Cys mutants and their
    labeled derivatives.
  • fig. S3. Test of protein oligomerization using MB analysis on the labeled species.
  • fig. S4. Calculation of the donor-acceptor distances using FPS software.
  • fig. S5. Analysis of the RNAP holoenzyme assembly by the ssFCS.
  • fig. S6. Interaction of RNAP with an us-fork template.
  • table S1. Apparent distances (Rap) between domains σ2 and σ4 determined in ensemble LRET experiments.
  • table S2. Summary of the donor-acceptor distances for σ70 C442-C579.
  • table S3. Summary of the donor-acceptor distances for σB C151-C292.
  • Reference (40)

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