Science Advances

This PDF file includes:

• fig. S1. Incorporation of the fluorescent dyes into σ⁷⁰ and σ^B subunits.
  
• fig. S2. Activity of the σ⁷⁰ and σ^B double-Cys mutants and their
  labeled derivatives.
  
• fig. S3. Test of protein oligomerization using MB analysis on the labeled species.
  
• fig. S4. Calculation of the donor-acceptor distances using FPS software.
  
• fig. S5. Analysis of the RNAP holoenzyme assembly by the ssFCS.
  
• fig. S6. Interaction of RNAP with an us-fork template.
  
• table S1. Apparent distances (R_ap) between domains σ2 and σ4 determined in ensemble LRET experiments.
  
• table S2. Summary of the donor-acceptor distances for σ⁷⁰ C442-C579.
  
• table S3. Summary of the donor-acceptor distances for σ^B C151-C292.
  
• Reference (40)

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