Science Advances

Supplementary Materials

This PDF file includes:

  • fig. S1. Parasite localization in mixed stage infections and gametocyte sublocalization to BM subcompartments.
  • fig. S2. Parasite clearance, homing, and vascular leakage in BM.
  • fig. S3. Dynamics of vascular leakage during infection.
  • fig. S4. Leakage induced by transmigration and sequestration.
  • fig. S5. Mature gametocyte distribution and mobility.
  • Legends for movies S1 to S13
  • Legend for table S1

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Other Supplementary Material for this manuscript includes the following:

  • movie S1 (.avi format). Intravital imaging of BM in a Balb/c mouse infected with P. berghei ANKA mCherryHsp70 and intravenously injected with 70-kDa FITC-labeled dextran 24 hours after infection.
  • movie S2 (.avi format). Single P. berghei mCherry gametocyte moving against the blood flow in a C57BL/6 mouse intravenously injected with FITC-dextran (at 24 hours after infection).
  • movie S3 (.avi format). Compiled movie set of transmigrating gametocyte events.
  • movie S4 (.avi format). Compiled movie sets of gametocyte mobility in UBC-GFP mice.
  • movie S5 (.avi format). Multiple circulating and static purified gametocytes in the sinusoids (S), parenchyma (P), and vasculature (IV) of a UBC-GFP mouse.
  • movie S6 (.avi format). Fast circulation of mature gametocytes within the BM vasculature of a UBC-GFP C57BL/6 mouse.
  • movie S7 (.avi format). Fast circulation of mature gametocytes within the spleen vasculature of a UBC-GFP transgenic mouse.
  • movie S8 (.avi format). Multiple circulating and static purified gametocytes in the sinusoids (S), parenchyma (P), and vasculature (IV) of a UBC-GFP mouse.
  • movie S9 (.avi format). Multiple circulating and static purified gametocytes in the sinusoids (S), parenchyma (P), and vasculature (IV) of a UBC-GFP mouse.
  • movie S10 (.avi format). Multiple circulating and static purified gametocytes in the sinusoids (S), parenchyma (P), and vasculature (IV) of a UBC-GFP mouse.
  • movie S11 (.avi format). Multiple circulating and static purified gametocytes in the sinusoids (S), parenchyma (P), and vasculature (IV) of a UBC-GFP mouse.
  • movie S12 (.avi format). Multiple fast-circulating gametocytes in a vascular tree of the spleen of a UBC-GFP mouse.
  • movie S13 (.mov format). Leukocyte motility, crawling adhesion, diapedesis (image center and top), and accumulation at the vessel wall (image bottom) of a Lys-GFP control mouse.
  • table S1 (Microsoft Excel format). This table contains the normalized expression values across all the 456 genes included in the NanoString expression array.

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