Science Advances

Supplementary Materials

This PDF file includes:

  • fig. S1. Swimming motility of Leptospira.
  • fig. S2. Example kymographs of Leptospira cells crawling without slip.
  • fig. S3. WT and “unbent” mutant (ΔfcpA) strains of Leptospira.
  • fig. S4. Example kymographs of ΔfcpA mutant cells crawling without slip.
  • fig. S5. Movement of a microbead attached to the cell surface.
  • fig. S6. Movement of a microbead attached to the cell surface.
  • fig. S7. Kymographs of Leptospira cells labeled with Ab-LPS–coated beads or noncoated beads.
  • fig. S8. Relationship between swimming speed and bead movement.
  • fig. S9. Crawling motility of the pathogenic Leptospira.

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Other Supplementary Material for this manuscript includes the following:

  • movie S1 (.avi format). A L. biflexa cell swimming in a 10% Ficoll solution.
  • movie S2 (.avi format). WT L. biflexa cells crawling on a glass surface.
  • movie S3 (.avi format). Effect of an anti-LPS antibody on crawling.
  • movie S4 (.avi format). ΔfcpA mutant cells crawling on a glass surface.
  • movie S5 (.avi format). Effect of CCCP on Leptospira crawling.
  • movie S6 (.avi format). Fluorescent observation of the Leptospira outer membrane using Cy3-NHS.
  • movie S7 (.avi format). Movement of a small bead aggregate on the Leptospira cell body.
  • movie S8 (.avi format). Movement of a single 200-nm bead on the Leptospira cell body.
  • movie S9 (.avi format). Movement of a large bead aggregate on the Leptospira cell body.

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