Science Advances

Supplementary Materials

This PDF file includes:

  • fig. S1. Identification of copurified protein with LsrK.
  • fig. S2. SEC-MALS analysis of the purified LsrK/HPr protein complex.
  • fig. S3. Electron density maps in the region of the bound HPr, ATP, and ADP.
  • fig. S4. Monitoring the stability of the synthesized p-15NHPr using HSQC experiment.
  • table S1. X-ray data collection and refinement statistics.
  • table S2. Strains and plasmids used for Miller assay.
  • table S3. Primer sequences for the in vivo studies of the wild-type and mutant HPr proteins.

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