Science Advances

Supplementary Materials

This PDF file includes:

  • Supplementary Text
  • Supplementary Methods
  • fig. S1. Photographs and Raman spectra of methane hydrate and a seismic profile of KMV#5.
  • fig. S2. Thermal gradient based on the temperatures measured in situ at KMV#5 and IODP site C0009.
  • fig. S3. Chloride concentrations versus stable isotopic compositions of water.
  • fig. S4. Depth profile of the estimated methane hydrate saturation in pore space based on δ18O and δD.
  • fig. S5. Average linkage clustering analysis based on the Bray-Curtis dissimilarity distance of 16S rRNA genes.
  • fig. S6. Community network analysis based on Spearman’s correlation coefficient.
  • fig. S7. Characteristics of the isolated methanogenic archaeon strain 1H1.
  • fig. S8. Chemical and stable isotopic compositions of hydrocarbon gases in the Nankai Trough area.
  • fig. S9. Mixing curves of Δ13CH3D between biogenic and thermogenic methane end-members consistent with a final clumped isotopologue temperature of 30°C for the resultant mixture.
  • References (61–79)

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Other Supplementary Material for this manuscript includes the following:

  • table S1 (Microsoft Excel format). Geochemical data from KMV#5 analyzed in this study.
  • table S2 (Microsoft Excel format). δ13C-CH4, δD-CH4, and Δ13CH3D temperature of Hybrid-PCS sediment core samples.
  • table S3 (Microsoft Excel format). Production test of gasses from Hybrid-PCS sediment core samples.
  • table S4 (Microsoft Excel format). Cell concentration in sediment core samples from KMV#5.
  • table S5 (Microsoft Excel format). Diversity indices of microbial communities in sediment core samples from KMV#5 based on 16S rRNA gene sequence analysis.
  • table S6 (Microsoft Excel format). Activity of methanogenesis, acetogenesis, and hydrogenase based on radiotracer incubation analyses.
  • table S7 (Microsoft Excel format). Concentration of archaeal core and IPLs.
  • table S8 (Microsoft Excel format). Thermogenic and biogenic end-member values for mixing calculation.

Download Tables S1 to S8

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