Science Advances

Supplementary Materials

This PDF file includes:

  • Supplementary Materials and Methods
  • fig. S1. Comparison of homozygous Arc-PBS mouse to WT mouse.
  • fig. S2. PCP binding to PBS-tagged mRNAs does not alter mRNA integrity.
  • fig. S3. Persistent synchronized Ca2+ spikes after bicuculline washout.
  • fig. S4. Synchronized Ca2+ spikes during bicuculline incubation.
  • fig. S5. Plot of Ca2+ activity and Arc transcription dynamics.
  • fig. S6. Comparison of somatic Ca2+ spikes between neurons with and without Arc transcription within 30 min after bicuculline stimulation.
  • table S1. Sequence of the probes used for smFISH against the Arc-CDS and PBS linkers (PBS).
  • Legends for movies S1 to S4
  • Reference (61)

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Other Supplementary Material for this manuscript includes the following:

  • movie S1 (.mp4 format). Immediate early activation of transcription from PBS-tagged Arc allele.
  • movie S2 (.mp4 format). Ca2+ activity of neurons induced by bicuculline stimulation.
  • movie S3 (.mp4 format). Arc transcription dynamics induced by bicuculline stimulation.
  • movie S4 (.mp4 format). Dendritic transport of Arc mRNA.

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