Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. Diversity of the fluorescent protein library facilitates evolution curriculum.
  • Fig. S2. Fluorescent protein library expresses with soluble, full-length products observed by SDS-PAGE and autoradiogram.
  • Fig. S3. FD-CF reactions tolerate a range of incubation temperatures.
  • Fig. S4. DNA template is not limiting for in vitro sfGFP synthesis due to relatively high initial rates of protein synthesis.
  • Fig. S5. Orange and yellow filters enable imaging of diverse fluorescent proteins in portable imagers.
  • Fig. S6. FD-CF reactions can be run in a laboratory-free environment using low-cost, portable imagers and incubators.
  • Fig. S7. Standard curves for converting fluorescence to protein concentrations.
  • Table S1. Cost analysis of portable imagers and incubators.
  • Table S2. Cost analysis for BioBits™ Bright.
  • Table S3. Cost analysis of FD-CF reactions.
  • Table S4. Plasmids used in this study.
  • Legends for curricula S1 to S5
  • Legend for data S1
  • Legend for folder S1

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Other Supplementary Material for this manuscript includes the following:

  • Curriculum S1 (Microsoft Word format). Let it glow!
  • Curriculum S2 (Microsoft Word format). What factors affect CFPS yields?
  • Curriculum S3 (Microsoft Word format). Synthetic biology: Looking to nature to engineer new designs.
  • Curriculum S4 (Microsoft Word format). How fast is it really?
  • Curriculum S5 (Microsoft Word format). Super power protein!
  • Data S1 (Microsoft Excel format). This file contains example student-generated fluorescence data from the tunable protein expression laboratory activity (Fig. 3) and includes time-course data for modeling protein synthesis as an enzymatic reaction with varying amounts of substrate (DNA template).
  • Folder S1 (.zip format). This folder contains FreeCAD files and circuit diagrams to enable user construction of portable imagers and incubators.

Files in this Data Supplement: