Science Advances

Supplementary Materials

This PDF file includes:

  • Supplementary Materials and Methods
  • Fig. S1. Direct cloning and expression of VHHs.
  • Fig. S2. VHHs block the interaction between the S1 protein and the MERS-CoV entry receptor DPP4.
  • Fig. S3. Amino acid sequences of VHH regions of anti–MERS-CoV spike VHHs.
  • Fig. S4. Phylogenetic tree of the amino acid sequences of the 46 MERS-CoV–neutralizing VHHs showing the corresponding neutralizing capacity of each VHH.
  • Fig. S5. Interaction of selected VHHs with recombinant MERS-CoV spike protein.
  • Fig. S6. Kinetics of VHH-1, VHH-4, VHH-83, and VHH-101 binding to MERS-CoV spike protein.
  • Fig. S7. Cross-competitive behavior of four different VHH-1, VHH-4, VHH-83, and VHH-101 determined using an Octet biosensor (ForteBio QK).
  • Fig. S8. Protective efficacy of MERS-CoV–specific VHHs in transgenic mice.
  • Table S1. Characteristics of MERS-CoV–specific VHHs.
  • Table S2. List of antibodies used in this study.
  • References (4752)

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