Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. Design diagram of the brick-like (TT motifs 1 to 3) object prepared using caDNAno.
  • Fig. S2. Exemplary negative-stained TEM micrographs of the brick-like object (TT motifs 1 to 4) in different buffers/solvents.
  • Fig. S3. Laser-scanned fluorescent image of a 2.0% agarose gel that was run in an ice-cooled water bath.
  • Fig. S4. Laser-scanned fluorescent image of a 2.0% agarose gel that was run in an ambient temperature water bath.
  • Fig. S5. Design diagram of the brick-like (TT motifs 1 to 4) object prepared using caDNAno.
  • Fig. S6. Laser-scanned fluorescent image of a 2.0% agarose gel placed in an ice-cooled water bath.
  • Fig. S7. Design diagram of the pointer object.
  • Fig. S8. Exemplary negative-stained TEM micrographs of the pointer object in different buffers/solvents.
  • Fig. S9. Laser-scanned fluorescent image of a 2.0% agarose gel placed in a water bath.
  • Fig. S10. Laser-scanned fluorescent image of a 2.0% agarose gel placed in a water bath.
  • Fig. S11. Laser-scanned fluorescent image of a 2.0% agarose gel placed in a water bath.
  • Fig. S12. Laser-scanned fluorescent image of a 2.0% agarose gel placed in an ice-cooled water bath.
  • Fig. S13. Laser-scanned fluorescent image of a 2.0% agarose gel placed in a water bath.
  • Fig. S14. Laser-scanned fluorescent image of a 2.0% agarose gel placed in a water bath.
  • Fig. S15. Laser-scanned fluorescent image of a 2.0% agarose gel placed in a water bath.
  • Fig. S16. Laser-scanned fluorescent image of a 2.0% agarose gel placed in a water bath.
  • Fig. S17. Cryo-EM data of the brick-like object with TT-motifs (1) – (3) before crosslinking in folding buffer.
  • Fig. S18. Cryo-EM data of the brick-like object with TT-motifs (1) – (3) after crosslinking in folding buffer.
  • Fig. S19. Cryo-EM data of the brick-like object with TT-motifs (1) – (3) after crosslinking in phosphate-buffered saline (PBS).
  • Fig. S20. Slice-by-slice visualization of cryo-EM maps determined from brick samples.
  • Fig. S21. Cryo-EM data of the brick-like object with TT-motifs (1) – (4) before crosslinking in folding buffer.
  • Fig. S22. Cryo-EM data of the brick-like object with TT-motifs (1) – (4) after crosslinking in folding buffer.
  • Fig. S23. Design diagram of the switch object.
  • Fig. S24. Design diagram of the polymerization brick object.
  • Legend for table S1
  • Legends for movies S1 to S6
  • Reference (70)

Download PDF

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Sequences of staple strands for all DNA objects used in this work.
  • Movie S1 (.mov format). Tomogram obtained from cryo-EM of the brick-like variant with thymines at all staple termini and with TT motifs at all crossover sites.
  • Movie S2 (.mov format). Corresponding movie to fig. S20 for the nonirradiated (uncross-linked) sample of the brick-like variant with thymines at all staple termini and with TT motifs at all crossover sites in folding buffer and in the presence of 5 mM MgCl2.
  • Movie S3 (.mov format). Corresponding movie to fig. S20 for the irradiated (cross-linked) sample of the brick-like variant with thymines at all staple termini and with TT motifs at all crossover sites in folding buffer and in the presence of 5 mM MgCl2.
  • Movie S4 (.mov format). Corresponding movie to fig. S20 for the irradiated (cross-linked) sample of the brick-like variant with thymines at all staple termini and with TT motifs at all crossover sites in PBS buffer and in the absence of MgCl2.
  • Movie S5 (.mov format). Corresponding movie to fig. S20 for the nonirradiated (uncross-linked) sample of the brick-like variant with thymines at all staple termini, with TT motifs at all crossover sites and additional 5-T loops in folding buffer and in the presence of 5 mM MgCl2.
  • Movie S6 (.mov format). Corresponding movie to fig. S20 for the irradiated (cross-linked) sample of the brick-like variant with thymines at all staple termini, with TT motifs at all crossover sites and additional 5-T loops in folding buffer and in the presence of 5 mM MgCl2.

Files in this Data Supplement: