Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. Structural analysis of OphA.
  • Fig. S2. pH rate profile of OphAΔC6-2h at different pH values (7.0 to 10.0).
  • Fig. S3. Experimental data for complexes.
  • Fig. S4. Superimposition of OphAΔC6 structures.
  • Fig. S5. Sequence alignment of the methyltransferase domain of OphA (Ompol1_2087).
  • Fig. S6. Mass analysis of all mutants discussed in the manuscript.
  • Fig. S7. All in vivo inactive mutants have similar inactive confirmations.
  • Fig. S8. MD simulations and QM calculations.
  • Fig. S9. Kinetic isotope effect and solvent viscosity effect studies of OphAΔC6-2h.
  • Fig. S10. Alternate register for substrate peptide.
  • Table S1. Crystallographic data.
  • Table S2. Occupancy of SAM/SFG and SAH in OphA variants.
  • References (7175)

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