Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. Bilayer-nanomesh structure and transmittance study.
  • Fig. S2. Bilayer-nanomesh microelectrode demonstration.
  • Fig. S3. Impedance results from different bilayer-nanomesh MEAs.
  • Fig. S4. Bench-top sine wave signal recording.
  • Fig. S5. Light-induced artifact characterization.
  • Fig. S6. Demonstration of artifact-free, ITO/PEDOT:PSS bilayer-nanomesh microelectrodes.
  • Fig. S7. Artifact rejection and wireless recording system.
  • Fig. S8. Artifact rejection using Au nanomesh microelectrode.
  • Fig. S9. Histology studies.
  • Fig. S10. In vivo transparency of MEA.
  • Fig. S11. Optical imaging underneath microelectrode.
  • Fig. S12. In vivo impedance measurement after implantation.
  • Fig. S13. Optimization of nanosphere lithography.
  • Legends for movies S1 to S5

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Wide-field epifluorescence of the Ca++ indicator GCaMP6s showing the activity in the superficial layers of the mouse visual cortex and the surrounding areas (30× faster than the real time).
  • Movie S2 (.mp4 format). Video-rate two-photon Ca++ imaging from the neurons of the layer 2/3 of the mouse visual cortex expressing the Ca++ indicator GCaMP6s (30× faster than the real time).
  • Movie S3 (.avi format). Correlation between the ΔF/F of Ca++ wide-field epifluorescence and the MEA recording (30× faster).
  • Movie S4 (.mp4 format). The correlated response of arousal (left), the map of the modulation of the power of the MEA recording in different electrophysiology frequency bands (center), and the ΔF/F of the two-photon Ca++ imaging (right) (3× faster than the real time).
  • Movie S5 (.mp4 format). Map of the modulation of the power of the MEA recording in the multi-unit band (300 Hz to 7 kHz) during the alternation of visual stimuli and isoluminous gray screen presentations (3× faster than the recording) in which evoked cortical activity (higher, color-coded in red) alternates with spontaneous cortical activity (lower, color-coded in green) based on the stimulus/nonstimulus presentation.

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