Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. ETV7 is expressed in pediatric malignancies.
  • Fig. S2. PI3K and PTK2 signaling and anti–p-tyr Western blot of cell lysate of vector and ETV7 mouse pre-B cells.
  • Fig. S3. ETV7 expression in human pediatric tumor xenografts.
  • Fig. S4. Sequence comparison of ETV6 and ETV7 PNT domains and the position of Δ9, Δ27, Δ120/Δ115, Δ120/Δ159/+60 mutations in the ETV7 PNT domain, and the KALK mutation in the ETV7 ETS domain.
  • Fig. S5. mTOR localization and assembly.
  • Fig. S6. Effects of mTOR inhibitors and mTORC3 loss on mTOR signaling.
  • Fig. S7. Effect of ALK inhibition on p-AKTSer473 and relative FK506-binding protein expression in Karpas-299 cells.
  • Fig. S8. mTORC3 kinase is insensitive to Raptor or Rictor knockdown or Rictor knockout.
  • Fig. S9. ERMS-specific markers in Ptch+/−/ETVTG+/− tumors are preserved in Ptch+/−/ETVTG+/− cell lines.
  • Fig. S10. Whole phospho-p70S6KThr389 and p70S6K Western blots relating to Figs. 1C and 5D.
  • Table S1. Expression effects of ETV7.

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Induction of non-targeting ETV7shRNA in human DAOY medulloblastoma cells.
  • Movie S2 (.mp4 format). Induction of targeting ETV7shRNA in human DAOY medulloblastoma cells.

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