Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. Examples of mutant spectra used to assign individual methionine and valine correlations.
  • Fig. S2. Cross-validation of the valine and methionine methyl group assignments.
  • Fig. S3. Interaction of hsp60 with different nucleotides.
  • Fig. S4. Conformational changes of hsp60 induced by a nonhydrolyzable ATP analog (App-NHp) investigated by EM and NMR.
  • Fig. S5. NMR characterization of the HEWL thermal unfolding without/with hsp60.
  • Fig. S6. Translational diffusion properties characterized by diffusion-ordered NMR spectroscopy.
  • Fig. S7. Determination of HEWL binding site on hsp60 using PRE experiments.
  • Fig. S8. Refolding of HEWL by ATP-powered chaperonin.
  • Fig. S9. Real-time NMR study of hsp60 functional cycle without client protein.
  • Fig. S10. Population of active hsp60 in ATP/ADP/apo state and closed/open state.
  • References (44, 45)

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