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Supplementary Materials

The PDF file includes:

  • Fig. S1. Structural changes taking place and chemical shift restraints.
  • Fig. S2. Strips from 1H-1H-NOESY-1H-15N-HSQC spectra showing resonances emanating from residues 1074 and 1075 of the respective CID domains.
  • Fig. S3. Strips from 1H-1H-NOESY 1H-15N-HSQC spectra showing resonances emanating from residues 1076 and 1077 of the CID domains.
  • Fig. S4. 15N relaxation data for the bound CID and NCBD domains and amide secondary chemical shifts of the complexes.
  • Fig. S5. Backbone subnanosecond motions and ΔCp for the three CID/NCBD complexes.
  • Fig. S6. Frustration in the CID/NCBD complexes without electrostatics.
  • Fig. S7. 1H-15N-HSQC correlation spectra for bound and free CID and NCBD domains and the number of restraints in the structure determination as a function of amino acid residue.
  • Fig. S8. Restraints used for structure calculations and refinement.
  • Fig. S9. Urea and temperature denaturation experiments and Coulomb surfaces of the ancestral and extant CID/NCBD complexes.
  • Legend for table S1

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). ITC parameters and intermolecular NOEs.

Files in this Data Supplement: