Science Advances

Supplementary Materials

The PDF file includes:

  • Supplementary Text
  • Fig. S1. Concept models of optic-cup formation and epithelial bending.
  • Fig. S2. Versatile 3D vertex model describing multicellular dynamics in 3D space.
  • Fig. S3. Quantification of optic-cup morphogenesis.
  • Fig. S4. Quantitative simulations of optic-cup morphogenesis for all of the unknown parameters.
  • Fig. S5. Comparison between in silico and ex vivo optic-cup formation under perturbations.
  • Fig. S6. Actomyosin activities in in vivo and ex vivo optic-cup morphogenesis.
  • Table S1. Main symbols used in the 3D vertex model of optic-cup formation.
  • Table S2. Standard physical parameter values of cell states in computational simulations of optic-cup formation.
  • Table S3. Standard physical parameter values of boundary regions in computational simulations of optic-cup formation.
  • Table S4. Standard physical parameter values of cell behaviors used in computational simulations of optic-cup formation.
  • Table S5. Varied physical parameter values obtained from computational simulations of optic-cup formation.

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Hinged cell shape at the NR-RPE boundary in the in vitro optic cup.
  • Movie S2 (.mp4 format). In silico recapitulation of optic-cup morphogenesis using the versatile 3D vertex model.
  • Movie S3 (.mp4 format). Cell proliferation, constriction, and apoptosis in in silico optic-cup formation.
  • Movie S4 (.mp4 format). Dependence of in silico optic-cup morphogenesis on cell heightening, proliferation, apoptosis, and differentiation.
  • Movie S5 (.mp4 format). Dependence of in silico optic-cup morphogenesis on formation of spontaneous curvature of NR.
  • Movie S6 (.mp4 format). Pharmacological assays of actomyosin activities in vitro.
  • Movie S7 (.mp4 format). Dependence of in silico optic-cup morphogenesis on apical and lateral cell constrictions.
  • Movie S8 (.mp4 format). Lateral cell constrictions in vitro.
  • Movie S9 (.mp4 format). Characteristic alignment of intracellular actin fibers along the apicobasal axis in vitro.
  • Movie S10 (.mp4 format). Elastic and plastic responses of in vitro neuroepithelium to mechanical stimuli.
  • Movie S11 (.mp4 format). Calcium response to shear stress on the basal surface in vitro.
  • Movie S12 (.mp4 format). Lateral constrictions triggered by local up-regulation of intracellular calcium concentration in vitro.
  • Movie S13 (.mp4 format). In silico recapitulation of optic-cup morphogenesis with strain-triggered lateral constriction.

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