Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. G9a, SET7/9, and SMYD2 K-OPL substrate selectivity profiles.
  • Fig. S2. G9a, SET7/9, and SMYD2 enzyme assays.
  • Fig. S3. MS/MS analysis of G9a, SET7/9, and SMYD2 reaction products.
  • Fig. S4. Density map for SMYD2-SAH-GWKLNleSKRG structure and comparison of peptide conformation from previous structures.
  • Fig. S5. In vitro SMYD2 assays with protein substrates.
  • Fig. S6. Liquid chromatography (LC)–MS/MS analysis of recombinant PER2.
  • Table S1. Crystallographic data and refinement statistics.

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Other Supplementary Material for this manuscript includes the following:

  • Table S2 (Microsoft Excel format). LoB scores for the human proteome based off SMYD2 K-OPL selectivity profile.
  • Table S3 (Microsoft Excel format). Missense mutations predicted to affect SMYD2 lysine methylation signaling.
  • Table S4 (Microsoft Excel format). Recombinant PER2 peptides identified by LC-MS/MS.

Files in this Data Supplement: