Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. Comparison of fluorescence preservation of FPs and chemical fluorescent tracers between FDISCO and other clearing methods.
  • Fig. S2. Applicability of low temperature for increasing the endogenous fluorescence preservation of the BABB and uDISCO methods.
  • Fig. S3. Clearing performance of FDISCO for whole organs and whole body.
  • Fig. S4. Fluorescence imaging of the spinal cord after FDISCO, 3DISCO, and uDISCO clearing in Thy1-GFP-M mice.
  • Fig. S5. Comparison of whole-brain imaging for Thy1-YFP-H mouse brains after FDISCO, 3DISCO, and uDISCO clearing.
  • Fig. S6. LSFM imaging of the tdTomato-labeled whole brain after FDISCO clearing.
  • Fig. S7. Fluorescence of RV-DsRed–labeled mouse brains cleared by FDISCO, 3DISCO, and uDISCO.
  • Fig. S8. 3D visualization of the PRV-labeled neuronal projections in the brain by FDISCO clearing and LSFM imaging.
  • Fig. S9. 3D visualization of AAV-labeled descending motor axons from the primary motor cortex by FDISCO clearing.
  • Table S1. FDISCO protocols for various tissues.
  • Legends for movies S1 to S6

Download PDF

Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Imaging of FDISCO-cleared mouse brain (Thy1-GFP-M).
  • Movie S2 (.mp4 format). 3D reconstruction and rendering of nerve branches and motor endplates in the gastrocnemius muscle.
  • Movie S3 (.mp4 format). 3D visualization of the vasculature in the whole brain.
  • Movie S4 (.mp4 format). Reconstruction of the glomeruli in the intact kidney.
  • Movie S5 (.mp4 format). 3D visualization of the neurons projecting to VTA by RV injection.
  • Movie S6 (.mp4 format). Tracing of descending motor axons from both the left and right primary motor cortices by AAV labeling.

Files in this Data Supplement: