Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. Distribution of active and repressive epigenetic marks at breakpoint of WT and T(2;3)enEs/enX31.
  • Fig. S2. Distribution of active and repressive marks at ectopic sites.
  • Fig. S3. Expression of HA-En and HA-EnSTOP from HAen79 or HAen79STOP.
  • Fig. S4. en80 flies are homozygous viable and fertile and express En correctly.
  • Fig. S5. The 79-kb HA-en transgene sets up a 3D PcG domain at ectopic sites.
  • Fig. S6. H3K27me3 spreads beyond the HAen79 transgene to the marker genes.
  • Fig. S7. 4C interactions between the enPREs and flanking DNA in T(2;3)enEs/enX31 larvae.
  • Fig. S8. H3K27me3 distribution in en80∆1.5.
  • Fig. S9. RNA in situ of genes flanking the attP40 and attP3 sites.

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