Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. Enrichment of the mosquito feeding-deterrent active compounds via flash chromatography on reversed-phase C18 column.
  • Fig. S2. Enrichment of the mosquito feeding-deterrent active compounds via HPLC on an analytical reversed-phase C18 column.
  • Fig. S3. Elution profile of feeding-deterrent active fraction.
  • Fig. S4. MALDI-TOF MS analysis of HPLC-enriched feeding-deterrent Peak#3.
  • Fig. S5. Electrospray ionization–Orbitrap MS analysis of the HPLC-enriched deterrent active Xbu Peak#3 showing observed charged states.
  • Fig. S6. MS/MS spectra of doubly-charged molecular species at m/z 651.9 and 673.9.
  • Fig. S7. Amino acid analyses of purified feeding-deterrent fraction.
  • Fig. S8. Appearance of cotton cloths used in the Hemotek-based membrane-feeding assays.
  • Fig. S9. Typical example of Xbu streaked on LBTA (Luria broth agar supplemented with indicator stains bromothymol blue and triphenyltetrazolium chloride) plate.
  • Table S1. Mosquito feeding data used for Probit analysis to generate Tables 1 and 2.
  • Table S2. Results of the N-terminal Edman sequencing trial.
  • Legend for movie S1

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Video clip showing feeding response of mosquitoes with water control and at a 90% feeding-deterrence dose of Xbu Peak#3.

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