Science Advances

Supplementary Materials

The PDF file includes:

  • Table S1. Morphological parameters based on dendrite reconstruction.
  • Table S2. Comparison of males (M) versus females (F) pyramidal cell morphology.
  • Table S3. Microglia number and density (per mm3) and area (μm2) and volume (μm3).
  • Table S4. Volume quantifications (μm3) for all brain structures.
  • Fig. S1. Reconstructions of CA3 pyramidal neurons of E21 and P0 CTL rats.
  • Fig. S2. Reconstructions of CA3 pyramidal neurons of E21 and P0 VPA-treated rats.
  • Fig. S3. Nonapical pyramidal cell morphology alterations and cell position within the CA3 zone.
  • Fig. S4. Effect of birth on microglia density and volume in the dorsolateral striatum of CTL and VPA-treated rats.
  • Fig. S5. The iDISCO clearing method enables concurrent 3D volume analyses of brains of rat pups.
  • Fig. S6. Lateral ventricle changes among all conditions (E21 CTL, E21 VPA, P0 CTL, P0 VPA, and P0 VPA + BUM).
  • Fig. S7. Medial axis and distance map of hippocampus.

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Spot detection of hippocampal microglia and morphometric-based microglia analysis.
  • Movie S2 (.mp4 format). Imaris segmentation of all brain structures.

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