Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. LacY and YidC reconstituted into PE/PG phospholipid membranes.
  • Fig. S2. SecYEG and SecYEG-YidC fusion construct reconstituted into PE/PG phospholipid membranes.
  • Fig. S3. YidC, SecYEG, and SecYEG-YidC fusion constructs are functional in vivo.
  • Fig. S4. Coadsorption of YidC and LacY proteoliposomes.
  • Fig. S5. YidC facilitates random insertion and folding of LacY.
  • Fig. S6. Classification criteria of LacY misfolding and folding events.
  • Fig. S7. Completely unfolded and extracted LacY polypeptides cannot insert and fold into phospholipid membranes without assistance.
  • Fig. S8. SecYEG facilitates sequential insertion and folding of LacY.
  • Fig. S9. SecYEG and YidC together facilitate sequential insertion and folding of LacY.
  • Fig. S10. SecY-YidC fusion construct cleaved by the PreScission protease.

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