Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. Conceptual illustrations of the LNT platform.
  • Fig. S2. Characterization of nanoparticles.
  • Fig. S3. Diffusion of nanoparticles tethered to an SLB.
  • Fig. S4. Design and implementation of a Disassembly YES gate.
  • Fig. S5. Tethering of nanoparticles to an SLB.
  • Fig. S6. Image analysis pipeline.
  • Fig. S7. Scattering signal profiles of red, green, and blue nanoparticles.
  • Fig. S8. Nucleotide-level schematics of two-input logic gates.
  • Fig. S9. Design principles for nanoparticle logic gates.
  • Fig. S10. Modularity of a hairpin-based two-input Assembly AND gate.
  • Fig. S11. Uneven responses of a two-input Disassembly OR gate.
  • Fig. S12. A dual-rail NAND gate.
  • Fig. S13. Disassembly INHIBIT and six-input Disassembly gates.
  • Fig. S14. Complex multi-input Disassembly gates.
  • Fig. S15. Dark-field snapshots of two-layer AND-AND and AND-OR cascade circuits.
  • Fig. S16. Two-layer OR-AND and INHIBIT-AND cascade circuits.
  • Fig. S17. A two-layer OR-OR cascade circuit.
  • Fig. S18. A nanoparticle multiplexer circuit.
  • Fig. S19. Effect of DNA concentration on floater diffusion.
  • Table S1. Response rates of two-input logic gates.
  • Table S2. DNA sequences of thiolated strands used for functionalizing nanoparticles.
  • Table S3. DNA sequences and experimental conditions used in circuit operations.
  • Legends of Movies S1 to S13
  • Reference (47)

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Time-lapse dark-field imaging of a nanoparticle Assembly YES gate.
  • Movie S2 (.mp4 format). Time-lapse dark-field imaging of a nanoparticle Disassembly YES gate.
  • Movie S3 (.mp4 format). Receptor-only visualization of a dark-field movie.
  • Movie S4 (.mp4 format). Time-lapse dark-field imaging of a two-input Assembly AND gate.
  • Movie S5 (.mp4 format). Time-lapse dark-field imaging of a two-input Assembly OR gate.
  • Movie S6 (.mp4 format). Time-lapse dark-field imaging of a two-input Disassembly AND gate.
  • Movie S7 (.mp4 format). Time-lapse dark-field imaging of a two-input Disassembly OR gate.
  • Movie S8 (.mp4 format). Time-lapse dark-field imaging of a two-input Disassembly INHIBIT gate.
  • Movie S9 (.mp4 format). Time-lapse dark-field imaging of a six-input Disassembly gate.
  • Movie S10 (.mp4 format). Time-lapse dark-field imaging of a two-input Disassembly AND gate with three outputs.
  • Movie S11 (.mp4 format). Time-lapse dark-field imaging of a two-layer AND-AND cascade circuit.
  • Movie S12 (.mp4 format). Time-lapse dark-field imaging of a two-layer AND-OR cascade circuit.
  • Movie S13 (.mp4 format). Time-lapse dark-field imaging of a nanoparticle multiplexer circuit.

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