Science Advances

Supplementary Materials

This PDF file includes:

  • Supplementary Materials and Methods
  • Fig. S1. Infectious ZIKV can be detected in the serum at multiple time points after infection in mice.
  • Fig. S2. DENV-immune serum neutralizes DENV but not ZIKV.
  • Fig. S3. Reduced fetal size during ZIKV infection enhanced by DENV immunity.
  • Fig. S4. Additional control groups demonstrate the requirement of cross-reactive antibodies and ZIKV for enhanced microcephaly.
  • Fig. S5. Immunocompromised dams die during pregnancy because of ZIKV infection.
  • Fig. S6. Reduced cortical thickness in ZIKV-infected mice enhanced by maternal DENV immunity.
  • Fig. S7. Normalized ratio of cortical thickness to body mass for fetal mice.
  • Fig. S8. Confirmation of protein-level reduction of BRN1 and PAX6 in fetal brains during maternal ZIKV infection.
  • Fig. S9. ZIKV viral genome copies in pregnant versus nonpregnant female mice.
  • Fig. S10. ZIKV antigens detected in the fetal cortex.
  • Fig. S11. Flow cytometry gating strategy to identify syncytiotrophoblasts and fetal endothelial cells.
  • Fig. S12. IC antibody injection does not affect brain development in uninfected mice.
  • References (54, 55)

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