Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. Activated GC neurons after treatment of contextual fear, enriched environment exposure, or voluntary running, respectively.
  • Fig. S2. Unchanged total GFP+ NSCs and increased Ki67+ rNSCs and ANPs after running.
  • Fig. S3. Increased DCX+ cells and Tbr2+ cells in the hippocampus after voluntary running.
  • Fig. S4. Excited DG glutamatergic neurons but not GABAergic neurons in voluntary running.
  • Fig. S5. AAV-infected vGluT2+-specific DG neurons.
  • Fig. S6. c-Fos+ neurons in the DG region after CNO treatment in various doses.
  • Fig. S7. Effects of CNO control with various doses.
  • Fig. S8. Analysis of sequencing results.
  • Fig. S9. Ephrin-B3 ligand prevents proliferation/differentiation of aNSCs and their transition to neurons.
  • Fig. S10. EphB2 kinase–dependent signaling is required for the maintenance of quiescent rNSCs.
  • Legends for movies S1 to S3

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Voluntary running behavior of a mouse in the running wheel.
  • Movie S2 (.mp4 format). In vivo fiber photometry of Ca2+ signal of DG granule neurons during running trials.
  • Movie S3 (.mp4 format). 3D reconstruction of confocal images of rNSCs and GCs.

Files in this Data Supplement: