Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. Identification of the methyltransferase responsible for methylating Plk1.
  • Fig. S2. Validation of K209me1 or pT210 antibody recognition of various peptides and mutant cells.
  • Fig. S3. K209me1 of Plk1 antagonizes pT210.
  • Fig. S4. Generation of K209A and K209M knock-in Plk1 mutant cell lines.
  • Fig. S5. Plk1 K209M mutant slightly delays G2/M transition.
  • Fig. S6. Plk1 K209me1 does not affect the kinetochore tension on the metaphase onset.
  • Fig. S7. Plk1 K209me1 is required for DNA damage repair.
  • Fig. S8. Plk1 K209me1 is not required for the recruitment of DNA damage factors to DNA damage sites.
  • Legends for movies S1 to S4

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). A representative HeLa/RFP-H2B cell bearing wild-type Plk1 was recorded from nuclear breakdown to cytokinesis by time-lapse microscopy.
  • Movie S2 (.avi format). A representative HeLa/RFP-H2B cell bearing K209A mutant Plk1 was recorded from nuclear breakdown to cytokinesis by time-lapse microscopy.
  • Movie S3 (.avi format). A representative HeLa/RFP-H2B cell bearing K209M mutant Plk1 was recorded from nuclear breakdown to cytokinesis by time-lapse microscopy.
  • Movie S4 (.avi format). A population of HeLa/RFP-H2B cells bearing K209M mutant Plk1 was recorded from nuclear breakdown to cytokinesis by time-lapse microscopy.

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