Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. Generation of multipotent NCSC populations from multiple hPSC lines.
  • Fig. S2. Serum treatment directs iPSC-derived NCSCs toward mural cells.
  • Fig. S3. Supplemental analysis of hPSC-derived pericyte-like cells.
  • Fig. S4. Supplemental analysis of BMEC/hPSC-derived pericyte-like cell cocultures.
  • Fig. S5. Measurement of the effects of hPSC-derived pericyte-like cells on primary rat BMEC phenotypes.
  • Fig. S6. NCSCs maintained in E6-CSFD retain neural crest marker expression and do not develop pericyte marker expression.
  • Fig. S7. hPSC-derived pericyte-like cell assembly with brain endothelial cells.
  • Table S1. Antibody staining conditions and DNA primer sequences and running conditions.
  • Table S2. Pericyte-enriched genes identified by single-cell RNA-seq in mouse (40) with human homologs.

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Other Supplementary Material for this manuscript includes the following:

  • Table S3 (Microsoft Excel format). RNA-seq FPKM data for all samples.

Files in this Data Supplement: