Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. Protein expression of Bach1 in mouse embryos and generation of Bach1-KO and DoxBach1 hESCs.
  • Fig. S2. Usp7 is required for Bach1-induced stabilization of pluripotency proteins in hESCs.
  • Fig. S3. RNA-seq analysis for differential gene expression in WT and Bach1-KO hESCs.
  • Fig. S4. The role of Usp7 in the mesendodermal differentiation induced by Bach1 deficiency.
  • Fig. S5. The genome-wide distribution profiles of Bach1 binding sites and the Gene Ontology analysis.
  • Fig. S6. Depletion of Bach1 impairs recruitment of H3K27me3 and EZH2 to the mesendodermal gene.
  • Fig. S7. RNA-seq analysis for differential gene expression in WT and Bach1-KO hESCs on day 3 of differentiation.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1.1 (Microsoft Excel format). RNA-seq analysis of DE genes in WT and Bach1-KO hESCs (day 0).
  • Table S1.2 (Microsoft Excel format). The up-regulated genes associated with cell differentiation in Bach1-KO hESCs (day 0).
  • Table S1.3 (Microsoft Excel format). RNA-seq analysis of DE genes on day 3 of EB differentiation of WT hESCs and Bach1-KO hESCs.
  • Table S2.1 (Microsoft Excel format). Primers used for CRISPR sgRNA and off-target.
  • Table S2.2 (Microsoft Excel format). Primers used for plasmids construction and reporters.
  • Table S2.3 (Microsoft Excel format). Primers used for qRT-PCR.
  • Table S2.4 (Microsoft Excel format). Primers used for Lv-Con and Lv-Bach1 shRNAs.
  • Table S2.5 (Microsoft Excel format). The sequences of siRNAs.
  • Table S2.6 (Microsoft Excel format). Primers used for ChIP-qPCR.

Files in this Data Supplement: