Science Advances

Supplementary Materials

This PDF file includes:

  • Table S1. MANT-GDP dissociation rates of Rac1.
  • Fig. S1. 1H-13C HMQC spectra of the wild-type Rac1 and the T35A mutant.
  • Fig. S2. 1H-15N TROSY spectra of the wild-type Rac1 and the P29S mutant.
  • Fig. S3. CPMG RD analyses of the wild-type Rac1.
  • Fig. S4. Summary of the global fitted results of the CPMG RD and HSQC/HMQC analyses.
  • Fig. S5. Summary of the intramolecular PRE experiments.
  • Fig. S6. Summary of the global fitted results of the CPMG RD and HSQC/HMQC analyses of the Cys-less mutant of Rac1.
  • Fig. S7. Comparison between the structures of states A and B obtained from the two-state ensemble structural calculations.
  • Fig. S8. MD simulations of Rac1.
  • Fig. S9. Mg2+ affinity and NMR spectra of the R66E mutant of Rac1.

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