Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. Hydrogel height controls O2 gradients.
  • Fig. S2. Cluster size over time.
  • Fig. S3. Low O2 tension, rather than diffusional limitations or nutrient deprivation, facilitates cluster formation.
  • Fig. S4. Cluster formation is HIF independent and ROS-mediated.
  • Fig. S5. Experimental setup for time-lapse monitoring of increases in fluorescence upon proteolytic degradation of DQ-gelatin.
  • Fig. S6. Protease activity and inhibition.
  • Fig. S7. Vascular sprouting from clusters.
  • Fig. S8. Dynamic matrix stiffening accelerates vascular network formation.
  • Fig. S9. Increased matrix viscoelasticity influences vascular network formation.
  • Fig. S10. In vivo O2 measurements.
  • Legends for movies S1 to S8

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Time lapse of ECFCs encapsulated under hypoxic conditions at z = 0 μm above the bottom of the plate.
  • Movie S2 (.avi format). Time lapse of ECFCs encapsulated under hypoxic conditions at z = 100 μm above the bottom of the plate.
  • Movie S3 (.avi format). Time lapse of ECFCs encapsulated under hypoxic conditions at z = 200 μm above the bottom of the plate.
  • Movie S4 (.avi format). Time lapse of ECFCs encapsulated under hypoxic conditions at z = 300 μm above the bottom of the plate.
  • Movie S5 (.avi format). Time lapse of ECFCs encapsulated under nonhypoxic conditions at z = 0 μm above the bottom of the plate.
  • Movie S6 (.avi format). Time lapse of ECFCs encapsulated under nonhypoxic conditions at z = 100 μm above the bottom of the plate.
  • Movie S7 (.avi format). Time lapse of ECFCs encapsulated under nonhypoxic conditions at z = 200 μm above the bottom of the plate.
  • Movie S8 (.avi format). Time lapse of ECFCs encapsulated under nonhypoxic conditions at z = 300 μm above the bottom of the plate.

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