Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. Intercalator tilting is inhibited when dye coverage is high.
  • Fig. S2. Summary of different modes of rotational motion.
  • Fig. S3. Definitions of coordinate systems and reference frames.
  • Fig. S4. The effect of wobble and tilt upon emission polarization.
  • Fig. S5. Three experimental configurations for measuring intercalated dye orientations.
  • Fig. S6. Simulated measurements corresponding to parameter sets {θ = 85°, α = 85°} and {θ = 53.6°, α = 39.1°}.
  • Fig. S7. Intuitive description of the differences between experimental configurations.
  • Fig. S8. Example ROI used for ensemble fluorescence polarization measurements.
  • Table S1. Accounting for energy transfer.
  • Section S1. Intercalators prevent peeling and bubble formation under low-salt conditions
  • Section S2. Relating the measured intercalator orientation to DNA structure
  • Section S3. Theoretical model: Tilt wobble and twirl
  • Section S4. Distinguishing wobble and tilt
  • Section S5. Why is it necessary to rotate the DNA?
  • Section S6. The effect of energy transfer upon emission polarization
  • Section S7. How rapidly do intercalators twirl?
  • Section S8. Rapidly toggling between x-/y-polarized illumination is equivalent to unpolarized excitation within the image plane
  • Section S9. Because of reduced intercalator affinity for S-DNA, tilting is only observed at the end of the OST
  • Section S10. ROI selection for ensemble fluorescence polarization measurements
  • References (4360)

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