Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. Cryo-EM analysis of 6hisHpUreI in closed conformation.
  • Fig. S2. Cryo-EM analysis of 6hisHpUreI in open conformation.
  • Fig. S3. Cryo-EM densities (mesh) of the six TM helices in 6hisHpUreI in the closed and open conformations superposed with their atomic models.
  • Fig. S4. Details of the cryo-EM densities overlaid with their atomic models (sticks) of the PLs and C terminus in the closed (orange) and open (cyan) conformations.
  • Fig. S5. Ordered lipids resolved in the cryoEM density maps.
  • Fig. S6. Data processing workflow used for the dataset recorded for the sample in the closed conformation.
  • Fig. S7. Data processing workflow used for the dataset recorded for the sample in the open conformation.
  • Fig. S8. Urea passage activity for wild-type HpUreI and mutants of 6hisHpUreI.
  • Fig. S9. Proposed filter model for urea fitted in the open conformation of 6hisHpUreI by using HADDOCK2.2.
  • Fig. S10. Sample quality evaluation for 6hisHpUreI in DDM other than amphipol.
  • Table S1. Statistics of the cryo-EM structures.
  • Legends for movies S1 to S5

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Cryo-EM density views of 6hisHpUreI in closed conformation.
  • Movie S2 (.mov format). Atomic model and density fit views of 6hisHpUreI in closed conformation.
  • Movie S3 (.mov format). Cryo-EM density views of 6hisHpUreI in open conformation.
  • Movie S4 (.mov format). Atomic model and density fit views of 6hisHpUreI in open conformation.
  • Movie S5 (.mov format). 6hisHpUreI closed and open conformations morph.

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