Science Advances

Supplementary Materials

The PDF file includes:

  • Supplementary Materials and Methods
  • Fig. S1. Schematic diagram of conjugation of polyanions to bonding of collagen fibrils.
  • Fig. S2. Snapshot of the simulation of the Ca2+, HPO42−, and PAA system.
  • Fig. S3. Conventional TEM of ruthenium red and uranyl acetate stained collagen fibrils.
  • Fig. S4. High performance liquid chromatography of HPAA-collagen in various extraction media.
  • Fig. S5. Particle size distribution of the mineralizing solution.
  • Fig. S6. Representative TEM images of different mineralization phase of HPAA-collagen.
  • Fig. S7. Conventional TEM examination of LPAA-collagen mineralized with CaP.
  • Fig. S8. Molecular dynamics simulation of several collagen fibrillar strctures containing bound HPAA molecules.
  • Fig. S9. Characterization of mineralized collagen fibrils.
  • Fig. S10. Biocompatibility evaluation of bare collagen sponges versus HPAA-collagen sponges.
  • Legends for movies S1 to S4

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Molecular dynamics simulation of aggregation of Ca2+ and HPO42− in the presence of PAA.
  • Movie S2 (.mp4 format). Early phase of intrafibrillar mineralization of HPAA-collagen.
  • Movie S3 (.mp4 format). Middle phase of intrafibrillar mineralization of HPAA-collagen.
  • Movie S4 (.mp4 format). Late phase of intrafibrillar mineralization of HPAA-collagen.

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