Science Advances

Supplementary Materials

This PDF file includes:

  • Table S1. The list of antibodies used in this study.
  • Table S2. Primer information for quantitative real-time fluorescence PCR analysis of expression of target genes.
  • Fig. S1. Specificity of the pT735-RalGAPα1 antibody.
  • Fig. S2. Effects of HFD/fatty acids on the RalGAPα1/β complex and RalA/B activities in skeletal muscle and L6 myotubes.
  • Fig. S3. Generation and characterization of the RalGAPα1Thr735Ala knock-in mice.
  • Fig. S4. Uptake of glucose and LCFAs and expression of genes for lipid metabolism in adipose tissues and primary adipocytes.
  • Fig. S5. Cell surface and total CD36 in L6 myotubes.
  • Fig. S6. Characterization of RalGAPα1-mKO mice.
  • Fig. S7. Mapping of interaction domains on RalGAPα1 and RalGAPβ.
  • Fig. S8. Expression, degradation, and interaction of RalGAPα1 and RalGAPβ in L6 myotubes expressing β-blockatide.

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