Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. Stability of VPS4B-treated CHMP2A-CHMP3 tubular structure over an extended period of time.
  • Fig. S2. Constriction time for 5 μM VPS4B-treated CHMP2A-CHMP3 tube in the presence of ATP Mg2+.
  • Fig. S3. Effect of VPS4B E235Q and I124E mutants on CHMP2A-CHMP3 tube remodeling.
  • Fig. S4. Snapshots of constricted tubes.
  • Fig. S5. Negative staining EM images of CHMP2A-CHMP3 tubes.
  • Fig. S6. Cryo-EM images of CHMP2A-CHMP3 tubes incubated with 50 μM VPS4B in the presence of 50 μM adenylyl-imidodiphosphate (AMP-PNP) Mg2+.
  • Fig. S7. Negative staining images of CHMP2A-CHMP3 tubes incubated with 5 μM VPS4B and 200 μM ATP Mg2+, indicating asymmetric and symmetric constriction sites, along with dome-like end cap formation at the constriction sites.
  • Legends for movies S1 to S10

Download PDF

Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). CHMP2A-CHMP3 tube immobilized on a membrane bilayer without VPS4B.
  • Movie S2 (.avi format). CHMP2A-CHMP3 tube immobilized on a membrane bilayer with 10 μM VPS4B.
  • Movie S3 (.avi format). CHMP2A-CHMP3 tubes immobilized on a membrane bilayer, preincubated with 10 μM VPS4B.
  • Movie S4 (.avi format). CHMP2A-CHMP3 tubes immobilized on a membrane bilayer, preincubated with 5 μM VPS4B.
  • Movie S5 (.avi format). CHMP2A-CHMP3 tubes immobilized on a membrane bilayer, preincubated with 10 μM VPS4B E235Q mutant and 200 μM ATP Mg2+.
  • Movie S6 (.avi format). CHMP2A-CHMP3 tubes immobilized on a membrane bilayer, preincubated with 10 μM VPS4B I124E and 200 μM ATP Mg2+.
  • Movie S7 (.avi format). Movie S7. CHMP2A-CHMP3 tubes immobilized on a membrane bilayer, preincubated with 10 μM VPS4B.
  • Movie S8 (.avi format). CHMP2A-CHMP3 tubes immobilized on a membrane bilayer, preincubated with 5 μM VPS4B.
  • Movie S9 (.avi format). CHMP2A-CHMP3 tubes immobilized on a membrane bilayer, preincubated with 3 μM VPS4B.
  • Movie S10 (.avi format). CHMP2A-CHMP3 tubes immobilized on membrane bilayer, preincubated with 1 μM VPS4B.

Files in this Data Supplement: