Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. Cryo-EM analysis of the whole 20S complex, the α-SNAP–SNARE subcomplex, and the NSF-D1D2 part.
  • Fig. S2. The flowchart for EM data processing.
  • Fig. S3. Representative raw gel images.
  • Fig. S4. Analyses of the expression levels of the wild-type α-SNAP and the mutants in the neurons.
  • Fig. S5. Sequence alignment of α-SNAP from different species.
  • Fig. S6. Effects of multiple point mutations of VAMP on the α-SNAP–dependent binding of NSF to the SNARE complex.
  • Fig. S7. Focused 3D classification of the α-SNAP–SNARE subcomplex together with the NSF N domain.
  • Fig. S8. The N-terminally deleted SNARE proteins can form the 20S complex.
  • Table S1. Cryo-EM data collection and refinement statistics.
  • Table S2. CXMS analysis of the 20S complex formed with either wild-type SNARE proteins or the N-terminally truncated SNARE proteins.
  • Table S3. The amino acid sequences of the NSF and SNARE proteins used in this study.

Download PDF

Files in this Data Supplement: