Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. Specificity of the FCA antibody.
  • Fig. S2. Complementation of coolair.
  • Fig. S3. Bait proteins for pull-down assay.
  • Fig. S4. Co-IP of FCA and CLF.
  • Fig. S5. Complementation of clf-29 with ProCLF:HA-CLF.
  • Fig. S6. Phenotype of the fca-9 clf-29 double mutant.
  • Fig. S7. CLF transcript level and CLF protein level in fca-9 and coolair plants.
  • Fig. S8. Co-IP of FCA and SSU72.
  • Fig. S9. Complementation of ssu72-1.
  • Fig. S10. A model showing that binding of nascent COOLAIR RNA by FCA is required for H3K27me3 and silencing of FLC.
  • Data file S1. Plasmids and primers.

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