Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. Flow cytometry.
  • Fig. S2. Alignment of 5′ ends of transcripts of A. ceratii showing SL and relict SL repeats.
  • Fig. S3. The distribution of intron sizes in A. ceratii genes.
  • Fig. S4. Expansion of gene numbers per metabolic pathway in A. ceratii.
  • Fig. S5. Phylogenetic analysis of prokaryotic and eukaryotic PKS and FAS.
  • Fig. S6. Predicted evolution of tetrapyrrole biosynthesis in A. ceratii and other dinoflagellates.
  • Fig. S7. Confocal microscopy images of A. ceratii and A. catenella as a control.
  • Fig. S8. CoxI phylogeny.
  • Fig. S9. Plot obtained by assembling the PE dataset including spike-in mitochondrial reads of P. polycephalum.
  • Fig. S10. Plot obtained by assembling the PE dataset including spike-in mitochondrial reads of P. polycephalum.
  • Fig. S11. Model of the mitochondrial respiratory chain in A. ceratii.
  • Table S1. Enriched gene families.
  • Table S2. Number of genes encoding transcription factors in genomes/transcriptomes of 48 genera.
  • Table S3. SL detected in the genome of A ceratii.
  • Table S4. Illumina RNAseq reads of A. ceratii at 6 and 96 hours during the infection.
  • Table S5. A list of enzymes involved in various metabolic pathways in the A. ceratii genome.
  • Table S6. Mitochondrion respiration assay.
  • Table S7. The search for components of the electron transport system of A. ceratii.
  • Table S8. Mitochondrial functions encoded in the genome of A. ceratii.
  • Table S9. Primers used in this study to amplify cox from A. ceratii cDNA.
  • Table S10. Primers used to amplify various shikimate pathway transcripts from A. ceratii cDNA.

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (.gz format). Contigs of the A. ceratii AT5.2 genome.
  • Data file S2 (.bz2 format). Scaffolds of the A. ceratii AT5.2 genome.
  • Data file S3 (.bz2 format). GFF of the A. ceratii AT5.2 genome.
  • Data file S4 (.gz format). Predicted protein sequences of the A. ceratii AT5.2 genome.
  • Data file S5 (.pdf format). Assembly statistics of the A. ceratii AT5.2 genome.
  • Data file S6 (Microsoft Excel format). Annotation table generated via Trinotate for the A. ceratii AT5.2 genome.

Files in this Data Supplement: