Science Advances

Supplementary Materials

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  • Fig. S1. Acylglucoses are not detected in trichome extracts of IL3-5, IL4-1, or IL11-3.
  • Fig. S2. Quantification of acylsugars in S. lycopersicum M82 and breeding lines containing S. pennellii LA0716 introgressions.
  • Fig. S3. Comparison of major acylsugars in BIL6521 and BIL6521 × BIL6180 F2 progeny using LC-MS.
  • Fig. S4. Mass spectra of major acylsugars S3:15, S3:22, G3:15, and G3:22 from BIL6521 × BIL6180—F2 lines, BIL6180, and BIL6521.
  • Fig. S5. Mutated genomic sequence of three homozygous spasff1 CRISPR-Cas9 lines.
  • Fig. S6. Acylsugars in BPI chromatograms of spasff1 and LA0716 plants.
  • Fig. S7. Comparison of acylsugars from IL3-5 and parental M82 using LC-MS.
  • Fig. S8. LC-MS analysis of P-type acylsucrose-producing S. lycopersicum BIL6180 stably transformed with proSpASFF1::SpASFF1.
  • Fig. S9. Mass spectra of G3:19-derived from SpASFF1 in vitro assay.
  • Fig. S10. SpASFF1 cleaves a purified P-type triacylsucrose but not unmodified sucrose while yeast invertase cleaves unmodified sucrose but not triacylsucrose.
  • Table S1. Annotation of acylsugars identified in BIL6521 and BIL6521 × BIL6180 F2 using LC-MS and collision-induced dissociation.
  • Table S2. Annotation of three GH candidates for SpASFF1 identified in the AG3.2.
  • Table S3. Primers/gBlocks/sgRNAs used in this study.

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