Science Advances

Supplementary Materials

The PDF file includes:

  • Table S1. Primers used for cloning in this work.
  • Table S2. Conditions used for cryo-EM data collection for all the cryo-EM maps in this work.
  • Table S3. Cryo-EM data refinement and validation statistics for all the cryo-EM maps in this work.
  • Table S4. Validation statistics for the atomic models for R2TP-ΔNT_structure3 and R2TP-ΔNT_structure4.
  • Fig. S1. Cryo-EM of truncated human R2TP (R2TP-ΔNT).
  • Fig. S2. Resolution estimation for cryo-EM structures obtained from the subtracted particles.
  • Fig. S3. High-resolution details in the cryo-EM maps.
  • Legends for movies S1 and S2

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Several views of the multibody refinement strategy, showing the motions of the PIH1D1 region bound to a DII domain of RUVBL2, with respect to the rest of the molecule.
  • Movie S2 (.mp4 format). Conformational changes in the RUVBL2 subunit, from a closed conformation to an open ADP-filled conformation to an open ADP-empty conformation.

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