Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. Stability of L620 fragment in the absence of P.
  • Fig. S2. Impact of kink abrogation and displacement on P functionality.
  • Fig. S3. Workflow of molecular dynamics simulation versus normal mode analysis and results of molecular dynamics simulations on wt and IPKI MD variants.
  • Fig. S4. Comparison of RMSF per residue for each chain and average over the four chains.
  • Fig. S5. Biochemical analysis of MD variants.
  • Fig. S6. AUC analysis of MD variants.
  • Fig. S7. Molecular dynamics simulations of V315N, V315I, and V315L MD variants.
  • Fig. S8. Lack of relationships between expression levels of the variants and their ability to support viral gene expression.
  • Table S1. Data collection and refinement statistics for the IPKI MD variant.
  • Table S2. Values for the main structural coiled-coil parameters of wt MD and of the IPKI variant as determined by TWISTER and RMSDs (Å) among the IPKI and the wt MD structures.
  • Legends for movies S1 and S2

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1A and Movie S1B (.mp4 format). Animations showing the low frequency collective motions also known as normal modes (NM) computed to extract large structure rearrangements of the wt and IPKI.
  • Movie S2A and Movie S2B (.mp4 format). Analysis of the curvature of the four helices along the different frames used to compute NM. The color gradient used for the animation is similar to the one used in Fig. 5C.

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