Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. Indel frequencies associated with ABEmax and engineered ABEmax mutants.
  • Fig. S2. DNA base editing and indel formation in HeLa cells from ABEmax and ABEmax mutants.
  • Fig. S3. DNA base editing, indel formation, and RNA editing in U2OS and K562 cells harvested 48 hours after nucleofection with ABEmax, ABEmax mutants, or Cas9(D10A).
  • Fig. S4. DNA base editing, indel formation, and RNA editing in HEK293T cells harvested 5 days after transfection with ABEmax or ABEmax mutants.
  • Fig. S5. Off-target DNA base editing associated with the HEK site 2 locus by ABEmax and ABEmax mutants.
  • Fig. S6. Off-target DNA base editing associated with the HEK site 3 locus by ABEmax and ABEmax mutants.
  • Fig. S7. Off-target DNA base editing associated with the HEK site 4 locus by ABEmax and ABEmax mutants.
  • Fig. S8. DNA base editing, indel formation, and RNA editing in HEK293T cells harvested 48 hours after transfection with ABEmax, ABEmaxAW, ABEmaxQW or ABEmax(TadA* A106V).
  • Fig. S9. A-to-I RNA editing across the transcriptome for ABEmax, ABEmaxAW, ABEmax(TadA E59A), and Cas9(D10A).
  • Fig. S10. Depiction of plasmid maps used in this study.
  • Table S1. Guide RNA sequences.
  • Table S2. Primers used for amplification of genomic DNA or cDNA for HTS.
  • Table S3. List of amplicon sequences used for alignment and analysis of HTS reads.
  • Table S4. List of primers used to amplify genomic off-target loci.
  • Table S5. List of interrogated off-target genomic loci (28), with guide RNA sequences and amplicons used for alignment.
  • Table S6. List of plasmid accession numbers from Addgene.

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