Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. Comparable numbers of microglia, Ly6Chigh and Ly6Clow populations, and gene expression levels of M1/M2 markers in macrophages between WT and IRF8−/− mice after SCI.
  • Fig. S2. The microglial IRF8–deficient does not affect CD68+ macrophage migration and functional recovery after SCI.
  • Fig. S3. Gating for EGFP+ peripheral blood monocytes.
  • Fig. S4. Macrophages infiltrated into the injured spinal cord by 3 dpi.
  • Fig. S5. Widespread IRF8−/− macrophage with few 5HT+ axons, leading to less demyelination after SCI.
  • Fig. S6. KEGG pathway analysis detected six cytokine-related pathways in analysis with RNA-seq data of 4, 7, and 14 dpi.
  • Fig. S7. IRF8 has no significant influence on expression levels of genes present in the cytokine-cytokine receptor interaction of KEGG pathway after SCI.
  • Fig. S8. IRF8 has no significant influence on expression levels of genes present in the nuclear factor κB signaling pathway of KEGG pathway after SCI.
  • Fig. S9. IRF8 has no significant influence on expression levels of genes present in the chemokine signaling pathway of KEGG pathway after SCI.
  • Fig. S10. IRF8 has no significant influence on expression levels of genes present in the TLR signaling pathway of KEGG pathway after SCI.
  • Fig. S11. IRF8 has no significant influence on expression levels of genes present in the transforming growth factor–β signaling pathway of KEGG pathway after SCI.
  • Fig. S12. IRF8 has no significant influence on expression levels of genes present in the TNF signaling pathway of KEGG pathway after SCI.
  • Fig. S13. Gene expression levels of cytokines in WT and IRF8−/− macrophages.
  • Fig. S14. Gene expression levels of cytokines in WT and IRF8−/− macrophages at 7 dpi.
  • Fig. S15. Gene expression levels of cytokines in WT and IRF8−/− macrophages at 14 dpi.
  • Fig. S16. The failure of centripetal migration of macrophages led to a wide range of neuronal loss after SCI.
  • Fig. S17. IRF8 did not significantly influence expression of C5a receptors of macrophages after SCI.
  • Fig. S18. EGFP+ macrophages at 1 hour after transplantation.
  • Fig. S19. Promoting IRF8 had no harmful effect on the systemic inflammatory response after SCI.
  • Fig. S20. TLR4 signaling of macrophages was activated after SCI.
  • Fig. S21. Sources of C5a in injured spinal cord.
  • Table S1. Primers used for qPCR.

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Chemotaxis assay of WT macrophages.
  • Movie S2 (.mp4 format). Chemotaxis assay of IRF8−/− macrophages.

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