Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. The feast-and-famine life cycle of E. coli.
  • Fig. S2. Time-of-death determination from PI fluorescence time series.
  • Fig. S3. Gompertz law of mortality informs and constraints biochemical models of aging and mortality in growth-arrested E. coli cells.
  • Fig. S4. Mortality statistics and goodness-of-fit of three independent experimental replicates for the three strains under study.
  • Fig. S5. Experimental repeatability and variances.
  • Fig. S6. Fabrication and implementation of the two-layer microfluidic chip.
  • Fig. S7. Noise removal from focusing fluctuations using regularized estimation of PI signal.
  • Fig. S8. Visualization and nonparametric statistics of variability among imaging positions within one experimental cohort.
  • Table S1. AIC-based model selection among GLMs for the three experimental replication cohorts (i = 1, 2, 3) of wild-type strain.
  • Table S2. AIC-based model selection among GLMs for the three experimental replication cohorts (i = 1, 2, 3) of ΔrssB strain.
  • Table S3. AIC-based model selection among GLMs for the three experimental replication cohorts (i = 1, 2, 3) of ΔrpoS strain.
  • Table S4. Testing for aging rate differences among the three strains using AIC and GLM.
  • Table S5. List of plasticwares that leach carbon-supplying contaminants and their nonleaching replacements.

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