Science Advances

Supplementary Materials

The PDF file includes:

  • Note S1. Genome assembly, mtDNA assembly, HaploMerger results, and BUSCO analysis.
  • Note S2. HiRise scaffolding and misassembly correction.
  • Note S3. Linkage group correction of misassemblies.
  • Legend for note S4
  • Note S5. RBH of orthologs.
  • Note S6. Whole-genome sequencing and RNA-based linkage map.
  • Note S7. MP spanning.
  • Note S8. Alignment between P. napi and P. rapae.
  • Note S9. Syntenic block support of scaffold joins.
  • Note S10. SNP generation from nextRAD library sequence.
  • Note S11. Comparison of P. napi versus B. mori collinear blocks within Lepidoptera genomes.
  • Note S12. Estimation of genomic rearrangements.
  • Note S13. Lepidopteran synteny cutoff selection.
  • Table S1. Read data used in assembly and analysis.
  • Legend for table S2
  • Table S3. Lepidopteran phylogeny calibrations.
  • Legend for table S4
  • Fig. S1. Chromosomal assembly validation figures.
  • Fig. S2. Alignment of P. rapae scaffolds to P. napi chromosomes.
  • Fig. S3. Example of criteria used to determine napi-like and mori-like joins.
  • Fig. S4. Permutation analysis of chromosomal terminal ends.
  • Fig. S5. Repetitive element distribution across chromosomes.
  • Fig. S6. Insert size distribution.
  • Fig. S7. The mtDNA of P. napi.
  • Fig. S8. Chronogram of lepidopteran genomes with node labels.
  • Fig. S9. Comparative assessment of genome assemblies and chromosomal evolution across, using a BLAST-like approach.
  • References (6985)

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Other Supplementary Material for this manuscript includes the following:

  • Note S4 (.zip format). Functional annotation summary.
  • Table S2 (Microsoft Excel format). Genomic data from Lepbase used in the comparative assessment of genome assemblies.
  • Table S4 (.tsv format). GO term enrichment in syntenic blocks.

Files in this Data Supplement: