Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. Dimension and shape of the coextrusion chip.
  • Fig. S2. Uniform laminin coating of the inner alginate wall.
  • Fig. S3. Diameter tube measurement using three coextrusion devices of different nozzle sizes.
  • Fig. S4. Immunostainings of CD31, fibrillin-1, and VE-cadherin were performed on vesseloids at day 1.
  • Fig. S5. Expression measurement of (i) basal and luminal markers, (ii) arterial and venous markers, and (iii) endothelial activation markers in regular culture conditions and in response to inflammatory stimuli.
  • Fig. S6. Quiescent cells at day 1 by loading the maximum of cells in the vesseloids.
  • Fig. S7. Hypoxia culture stabilizes the vesseloids.
  • Fig. S8. Vesseloid imaged by TEM at high magnification (×25,000 and ×50,000) after 1 day of culture.
  • Fig. S9. Lumenization with EC lining and formation of an external SMC layer in spherical capsules.
  • Legends for movies S1 to S4
  • Legend for data file S1

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). ECs and SMCs are dynamic after encapsulation.
  • Movie S2 (.avi format). The 20-kDa FITC-dextran perfusion.
  • Movie S3 (.avi format). ET-1 induces vesseloid contraction.
  • Movie S4 (.avi format). ET-1 induces intracellular calcium raise in both ECs and SMCs.
  • Data file S1 (.stl format). STL file of the coextrusion chip.

Files in this Data Supplement: