Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. Traditional translocation assays.
  • Fig. S2. Calibration of surface translocation assays.
  • Fig. S3. Surface translocation assays.
  • Fig. S4. AFM images of Sec translocases engaging precursors at the initial stage of activity.
  • Fig. S5. AFM Images of Sec translocases engaging precursors at the plateau stage of activity.
  • Fig. S6. Liposomes alone and liposomes containing just SecYEG.
  • Fig. S7. Translocase topography at lower protein-to-lipid (P:L) ratio.
  • Fig. S8. Representative Sec translocase monomers and dimers.
  • Fig. S9. SecA monomers and dimers without SecYEG.
  • Fig. S10. Simulated images of monomeric SecA and comparison to experiment.
  • Fig. S11. Simulated AFM images of SecA monomers in different conformations and dimeric SecA.
  • Fig. S12. Representative AFM images of Sec translocases with ATP.
  • Fig. S13. Representative AFM images of Sec translocases with ADP.
  • Fig. S14. Integrated volume histograms.
  • Fig. S15. Model optimization using Bayesian information criterion.

Download PDF

Files in this Data Supplement: