Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. Further in vitro characterization of the Lck biosensors.
  • Fig. S2. Characterization of Lck biosensors with different substrates in HeLa cells.
  • Fig. S3. Characterization of the ZapLck biosensor in JCam cells.
  • Fig. S4. Preactivation of Lck in Jurkat and PBMCs.
  • Fig. S5. Characterize the subcellular localization of mCherry-tagged Lck mutants, and the sensitivity of the ZapLck biosensor in detecting Lck activity compared with a Src biosensor.
  • Fig. S6. Phosphorylation level of endogenous ERK in T cells stimulated by CD3/CD28 antibody clusters.
  • Fig. S7. Activation of Lck kinase in JCam cells with different stimuli at different concentrations.
  • Fig. S8. CD28-only antibody did not trigger TCR signals.
  • Legends for movies S1 and S2

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). The dynamic change of ZapLck biosensor ECFP/FRET ratio signals under CD3/CD28 stimulation and PP1 inhibition in Jurkat or JCam cells with or without Lck or Lck mutants as indicated.
  • Movie S2 (.avi format). The dynamic change of ERK (NES) biosensor FRET/ECFP ratio signals under CD3/CD28 stimulation and PP1 inhibition in Jurkat or JCam cells with or without Lck or Lck mutants as indicated.

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