Science Advances

Supplementary Materials

The PDF file includes:

  • Fig. S1. TORC1 and TORC2 signaling pathways are regulated at the level of translation by Elongator.
  • Fig. S2. Setup of a genetic screen based on dual-color luciferase reporters with skewed codon content for lysine to identify regulators of the activity of Elongator.
  • Fig. S3. Synthetic data analysis of the primary (dual-color luciferase) and secondary (HA-Atf1) screens for regulators of Elonagtor.
  • Fig. S4. Effect of rapamycin and tor1/tor2 overexpression on the level of Elongator-dependent modification of uridine.
  • Fig. S5. Phosphorylation site of Elp4 is conserved in the fission yeast clade.
  • Fig. S6. Gad8 regulates Gsk3 activity in fission yeast.
  • Fig. S7. Specificity of the anti–phospo-Gad8 and anti–phospho-p70 S6 antibodies.
  • Fig. S8. Phosphorylation of Psk1 and Gad8 as reporter of TORC1 and TORC2 activities.
  • Fig. S9. A schematic view of the reciprocal control of TORC1/TORC2 and Elongator.
  • Legends for tables S1 to S4

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Results of the Chroma-Glo–based screen for regulators of Elongator.
  • Table S2 (Microsoft Excel format). Raw and processed data used to build Fig. 4C (sheet 1, TORC1 → TORC2) and Fig. 4D (sheet 2, TORC2 → TORC1).
  • Table S3 (Microsoft Excel format). Raw and processed data of the quantification of tRNA modifications used to build Figure 3A, left (sheet 1), 3A, right (sheet 2), and 4A (sheet 3).
  • Table S4 (Microsoft Excel format). List of strains used and generated in this study.

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